Choose your target carefully.
Even before you optimize your imaging protocol, says Jeff Peterson, vice president for biology at ViSen Medical, make sure you choose the right target for a particular biological question. For example, to image a breast tumor in vivo, appropriate targets might include matrix metalloproteases at the tumor's leading edge, the proliferative-phase nuclear protein Ki-67, the estrogen-linked cathepsin D, or a variety of other molecular markers. Each different target will provide different imaging results.
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Tips for in vivo imaging
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