Choose your substrate.
Basement membrane extract (BME) is
fine for studying tissue-specific differentiation, because it can
approximate the morphology and viscoelasticity of the tissues in
extracellular matrices in vivo, explains Valerie Weaver of the University
of California, San Francisco. If you're working on invasive tumors,
however, try collagen or a similar substrate, which better mimics the
interstitial matrix through which the tumors must migrate.
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Tips for setting up 3-D cell cultures
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