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Electroporation and the Single Cell
New technologies increase the resolution of electroporation
The Scientist 2004, 18(8):46
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To date, electroporation has largely been a brute-force way to deliver molecules such as DNA, RNA, and drugs into cells. The application of a voltage pulse creates in the cell membrane small, resealable pores whose size varies based on the amplitude and duration of the pulses. Whereas traditional electroporation devices permit only bulk intracellular delivery of molecules, companies are now introducing more sophisticated methods that allow users to manipulate individual cells and even to target specific regions of a cell.
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